Profluent is an AI-first protein design company. Founded in 2022, we develop deep generative models to design and validate novel, functional proteins to revolutionize biomedicine. Based in Emeryville, CA, we are backed by leading investors including Altimeter Capital, Bezos Expeditions, Spark Capital, Insight Partners, Air Street Capital, AIX Ventures, and Convergent Ventures, and have raised over $150M to date.

We are seeking a motivated and creative Research Associate to join our Therapeutic Translation Team. In collaboration with members of the Therapeutic Translation, Cellular Models and Automation teams, you will design, validate and execute high-throughput experiments to characterize and optimize therapeutic gene editing proteins. Your work will be foundational for expanding our gene editing toolbox and translating these advances to new therapeutic opportunities.

The ideal candidate will have experience with molecular biology, mammalian cell culture and NGS preparation workflows. This is an excellent opportunity to work cross-functionally with a diverse team of experts across machine learning, protein design, cell biology, gene editing and laboratory automation.

Responsibilities

Design, validate and execute end-to-end assays to measure on- and off-target activity of novel gene editing systems using a combination of targeted and genome-wide analytical methods
Maintain organized tracking of physical samples, reagents, consumables, and assay materials to support efficient high-throughput screening operations, including inventory management, sample traceability, procurement coordination
Compile and analyze data to inform critical go/no-go decisions, and clearly present conclusions and rationale to stakeholders across the organization
Collaborate with interdisciplinary teams to optimize engineered protein candidates for therapeutic applications
Stay up to date on the latest developments in gene editing technology

Qualifications

BS degree in Molecular Biology, Biochemistry, Cell Biology, or a related field with 2 - 4 years of experience or a MS degree within the same fields and 0 - 2 years of experience
Hands-on experience with molecular biology & cloning workflows
Hands-on experience with mammalian cell culture & maintenance
Conceptual understanding of illumina NGS library preparation & QC

Preferences

Experience with CRISPR and/or mammalian cell-based gene editing assays
Experience using Benchling as an electronic lab notebook and LIMS system
Experience with high throughput and/or pooled screening workflows
Experience with the design or execution of NGS-based assays and analysis workflows
Experience analyzing large experimental datasets (i.e. Python, SQL, JMP)
Understanding of the key concepts and challenges related to the field of genetic medicine, gene editing systems, DNA repair, etc.

What We Offer

High-growth opportunity with meaningful impact on the future of protein design
Competitive compensation package with equity participation
401(k) with a strong employer match
Comprehensive benefits including health/dental/vision insurance
Generous PTO policy and commitment to work-life balance
Professional development opportunities in a cutting-edge field at the intersection of AI and biology

Profluent Bio, Inc is an equal opportunity employer promoting diversity and inclusion in the workspace. We do not discriminate on the basis of race, color, religion, marital status, age, national origin, ancestry, physical or mental disability, medical conditions, veteran status, sexual orientation, gender (including gender identity and gender expression), sex (which includes pregnancy, childbirth, and breastfeeding), genetic information, taking or requesting statutorily protected leave, or any other basis protected by law.

Work Authorization Requirement

Applicants must have ongoing work authorization in the United States that does not require employer sponsorship. Sponsorship will not be provided now or at any time in the future for this position.

Employment Eligibility Verification

Legal authorization to work in the United States is required. In compliance with federal law, all persons hired must verify their identity and work eligibility and complete the required employment verification form upon hire.

Hiring Salary Range

$83,000—$104,000 USD

Research Associate II - Senior Research Associate I, Therapeutic Translation